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Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

Lentivectors

Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production.

 

    • Gas chromatography-mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions).

 

    • In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4.

 

    • After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 μg/g) than the sonication assay (120 μg/g), both giving far higher yields than the prick assay (19 μg/g).

 

    • Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR).

 

Hairy roots from cocultivation (six months-old) accumulated as much as 1086 μg/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varied with growth kinetics, being higher at the stationary phase.

 

This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating the potential for a future biotechnological production of chromenes.

 

Lentivectors
Lentivectors
Keywords: Agrobacterium rhizogenes A4; Helianthella quinquenervis; chromenes; encecalin; medicinal plant; secondary metabolites; sonication.

 

Alpha-bungarotoxin, CF405s

00002-100ug 100uG
EUR 161
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF680r

9-00003
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF680r

9-00003
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Intrinsic Type 1 Interferon (IFN1) Profile of Uncultured Human Bone Marrow CD45 low CD271 + Multipotential Stromal Cells (BM-MSCs): The Impact of Donor Age, Culture Expansion and IFNα and IFNβ Stimulation

 

Skeletal aging is associated with reduced proliferative potential of bone marrow (BM) multipotential stromal cells (MSCs). Recent data suggest the involvement of type 1 interferon (IFN1) signalling in hematopoietic stem cell (HSC) senescence.

 

Considering that BM-HSCs and BM-MSCs share the same BM niche, we investigated IFN1 expression profile in human BM-MSCs in relation to donor age, culture-expansion and IFN1 (α and β) stimulation.

 

    • Fluorescence-activated cell sorting was used to purify uncultured BM-MSCs from younger (19-41, n = 6) and older (59-89, n = 6) donors based on the CD45lowCD271+ phenotype, and hematopoietic-lineage cells (BM-HLCs, CD45+CD271) were used as controls.

 

    • Gene expression was analysed using integrated circuits arrays in sorted fractions as well as cultured/stimulated BM-MSCs and Y201/Y202 immortalised cell lines.

 

    • IFN1 stimulation led to BM-MSC growth arrest and upregulation of many IFN1-stimulated genes (ISGs), with IFNβ demonstrating stronger effects. Uncultured MSCs were characterised by a moderate-level ISG expression similar to Y201 cells.

 

Age-related changes in ISG expression were negligible in BM-MSCs compared to BM-HLCs, and intracellular reactive oxygen species (ROS) levels in BM-MSCs did not significantly correlate with donor age. Antiaging genes Klotho and SIRT6 correlated with more ISGs in BM-MSCs than in BM-HLCs.

 

In patients with osteoarthritis (OA), BM-MSCs expressed considerably lower levels of several ISGs, indicating that their IFN1 signature is affected in a pathological condition. In summary, BM-MSCs possess homeostatic IFN1 gene expression signature in health, which is sensitive to in vitro culture and external IFN1 stimulation. IFN signalling may facilitate in vivo BM-MSC responses to DNA damage and combating senescence and aberrant immune activation.

 

Keywords: aging; bone marrow; mesenchymal stromal cells; senescence; type 1 interferon.

 

Ozone disinfection kinetics of poliovirus 1 determined by cell culture assay, RT-qPCR, and Ethidium Monoazide qPCR Reduction in a Continuous Quench-Flow Reactor

 

Aims: A continuous quench-flow (CQF) reactor was developed to collect samples at the reaction times of less than one second. The reactor is applied to determine ozone disinfection kinetics of poliovirus and to study whether EMA-qPCR can assess the viral infectivity after ozone disinfection.

 

Methods: Ozone disinfection of poliovirus was conducted in the developed CQF, and the disinfection kinetics were tested in the range of 0.7 – 5.0 seconds at ozone concentration of 0.08 and 0.25 mg/L. Inactivation, damage on viral genome and damage on capsid integrity were determined by plaque assay, quantitative reverse transcription polymerase chain reaction (RT-qPCR), and ethidium monoazide treatment coupled with RT-qPCR (EMA-qPCR), respectively.

 

 

Postsynthetic Functionalization of DNA-Nanocomposites with Proteins Yields Bioinstructive Matrices for Cell Culture Applications

 

We report on the directed postsynthetic functionalization of soft DNA nanocomposite materials with proteins. Using the example of the functionalization of silica nanoparticle-modified DNA polymer materials with agonists or antagonists of the epidermal growth factor receptor EGFR cell membrane receptor, we demonstrate that hierarchically structured interfaces to living cells can be established.

 

Due to the modular design principle, even complex DNA nanostructures can also be integrated into the materials, thereby enabling the high-precision arrangement of ligands on the lower nanometer length scale. We believe that such complex biohybrid material systems can be used for new applications in biotechnology.

 

Keywords: DNA nanocomposites; DNA origami nanostructure; epidermal growth factor receptor; phosphorylation; protein-DNA conjugates.

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells

2-82573 1 Kit Ask for price

Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-46091 5 x 100 ml Ask for price

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-25073 5 x 100 ml Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-32074 5 x 100 ml Ask for price

Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80091 1 x 100 ml Ask for price

Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80188 1 x 100 ml Ask for price

Rat Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80280 1 x 100 ml Ask for price

Human Muscle PrimaCell2: Normal Smooth Muscle Cells

2-96092 1 Kit Ask for price

Mouse Muscle PrimaCell2: Normal Smooth Muscle Cells

2-82075 1 Kit Ask for price

Rat Muscle PrimaCell2: Normal Smooth Muscle Cells

2-82574 1 Kit Ask for price

Immortalized Chicken Skeletal Muscle Cells - SV40

T9007 1x106 cells / 1.0 ml Ask for price

Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47091 1 Set Ask for price

Human Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium

9-46092 5 x 100 ml Ask for price

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-26073 1 Set Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-33074 1 Set Ask for price

Human Adult Skeletal Muscle (Normal) Whole tissue lysate

HAL-1375 1 mg
EUR 524

cDNA from Human Adult Normal Tissue: Skeletal Muscle

C1234171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Adult Normal: Skeletal Muscle

T1234171 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle

T2234171 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA from Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Skeletal Muscle

C1734171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Soft Tissue Tissue Slide (Skeletal Muscle-Normal)

12-341-10um 10 um
EUR 201.5

Soft Tissue Tissue Slide (Skeletal Muscle-Normal)

12-341-4um 4 um
EUR 180.5

Human Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells

9-80092 1 x 100 ml Ask for price

Rat Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium

9-25074 5 x 100 ml Ask for price

Mouse Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium

9-32075 5 x 100 ml Ask for price

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells

2-96009 1 Kit Ask for price

Human Cervix PrimaCell3: Normal Cervical Smooth Muscle Cells

2-96046 1 Kit Ask for price

Human Esophagus PrimaCell2: Normal Esophageal Smooth Muscle Cells

2-96056 1 Kit Ask for price

Human Heart PrimaCell1: Normal Aortic Smooth Muscle Cells

2-96066 1 Kit Ask for price

Human Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells

2-96072 1 Kit Ask for price

Human Rectal PrimaCell: Normal Rectal Smooth Muscle Cells

2-96100 1 Kit Ask for price

Rat Bladder PrimaCell3: Normal Smooth Muscle Cells

2-82508 1 Kit Ask for price

Total Protein from Human Adult Normal Tissue: Skeletal Muscle

P1234171 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Adult Normal Tissue: Skeletal Muscle

P3234171 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue: Skeletal Muscle

R1234171-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) Normal Skeletal Muscle Whole tissue lysate

MRL-1247 1 mg
EUR 524

Monkey (Cyno.) Normal Skeletal Muscle Whole tissue lysate

MCL-1197 1 mg
EUR 524

cDNA from Monkey (Rhesus) Normal Tissue: Skeletal Muscle

C1534171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle

C1534171-Cy 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Skeletal Muscle

D1334171 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Skeletal Muscle

D1434171 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Mouse Normal Tissue: Skeletal Muscle

P1334171 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

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