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Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

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Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production.

 

    • Gas chromatography-mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions).

 

    • In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4.

 

    • After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 μg/g) than the sonication assay (120 μg/g), both giving far higher yields than the prick assay (19 μg/g).

 

    • Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR).

 

Hairy roots from cocultivation (six months-old) accumulated as much as 1086 μg/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varied with growth kinetics, being higher at the stationary phase.

 

This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating the potential for a future biotechnological production of chromenes.

 

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Keywords: Agrobacterium rhizogenes A4; Helianthella quinquenervis; chromenes; encecalin; medicinal plant; secondary metabolites; sonication.

 

Alpha-bungarotoxin, CF405s

00002-100ug 100uG
EUR 193.2
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF680r

9-00003
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF680r

9-00003
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF640r

9-00004
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF640r

9-00004
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF488a

9-00005
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF488a

9-00005
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
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  • 500uG
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Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
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  • 500uG
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Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 201.6
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 440.4
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 459.6
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 500.4
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 559.2
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 559.2
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 627.6
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 519.6
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
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  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
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  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 376.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 501.6
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 412.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 537.6
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 589.2
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 750
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 337.2
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 166.8
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 592.8
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 414
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 513.6
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 414
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 414
Description: Minimum order quantity: 1 unit of 300ST

Intrinsic Type 1 Interferon (IFN1) Profile of Uncultured Human Bone Marrow CD45 low CD271 + Multipotential Stromal Cells (BM-MSCs): The Impact of Donor Age, Culture Expansion and IFNα and IFNβ Stimulation

 

Skeletal aging is associated with reduced proliferative potential of bone marrow (BM) multipotential stromal cells (MSCs). Recent data suggest the involvement of type 1 interferon (IFN1) signalling in hematopoietic stem cell (HSC) senescence.

 

Considering that BM-HSCs and BM-MSCs share the same BM niche, we investigated IFN1 expression profile in human BM-MSCs in relation to donor age, culture-expansion and IFN1 (α and β) stimulation.

 

    • Fluorescence-activated cell sorting was used to purify uncultured BM-MSCs from younger (19-41, n = 6) and older (59-89, n = 6) donors based on the CD45lowCD271+ phenotype, and hematopoietic-lineage cells (BM-HLCs, CD45+CD271) were used as controls.

 

    • Gene expression was analysed using integrated circuits arrays in sorted fractions as well as cultured/stimulated BM-MSCs and Y201/Y202 immortalised cell lines.

 

    • IFN1 stimulation led to BM-MSC growth arrest and upregulation of many IFN1-stimulated genes (ISGs), with IFNβ demonstrating stronger effects. Uncultured MSCs were characterised by a moderate-level ISG expression similar to Y201 cells.

 

Age-related changes in ISG expression were negligible in BM-MSCs compared to BM-HLCs, and intracellular reactive oxygen species (ROS) levels in BM-MSCs did not significantly correlate with donor age. Antiaging genes Klotho and SIRT6 correlated with more ISGs in BM-MSCs than in BM-HLCs.

 

In patients with osteoarthritis (OA), BM-MSCs expressed considerably lower levels of several ISGs, indicating that their IFN1 signature is affected in a pathological condition. In summary, BM-MSCs possess homeostatic IFN1 gene expression signature in health, which is sensitive to in vitro culture and external IFN1 stimulation. IFN signalling may facilitate in vivo BM-MSC responses to DNA damage and combating senescence and aberrant immune activation.

 

Keywords: aging; bone marrow; mesenchymal stromal cells; senescence; type 1 interferon.

 

Ozone disinfection kinetics of poliovirus 1 determined by cell culture assay, RT-qPCR, and Ethidium Monoazide qPCR Reduction in a Continuous Quench-Flow Reactor

 

Aims: A continuous quench-flow (CQF) reactor was developed to collect samples at the reaction times of less than one second. The reactor is applied to determine ozone disinfection kinetics of poliovirus and to study whether EMA-qPCR can assess the viral infectivity after ozone disinfection.

 

Methods: Ozone disinfection of poliovirus was conducted in the developed CQF, and the disinfection kinetics were tested in the range of 0.7 – 5.0 seconds at ozone concentration of 0.08 and 0.25 mg/L. Inactivation, damage on viral genome and damage on capsid integrity were determined by plaque assay, quantitative reverse transcription polymerase chain reaction (RT-qPCR), and ethidium monoazide treatment coupled with RT-qPCR (EMA-qPCR), respectively.

 

 

Postsynthetic Functionalization of DNA-Nanocomposites with Proteins Yields Bioinstructive Matrices for Cell Culture Applications

 

We report on the directed postsynthetic functionalization of soft DNA nanocomposite materials with proteins. Using the example of the functionalization of silica nanoparticle-modified DNA polymer materials with agonists or antagonists of the epidermal growth factor receptor EGFR cell membrane receptor, we demonstrate that hierarchically structured interfaces to living cells can be established.

 

Due to the modular design principle, even complex DNA nanostructures can also be integrated into the materials, thereby enabling the high-precision arrangement of ligands on the lower nanometer length scale. We believe that such complex biohybrid material systems can be used for new applications in biotechnology.

 

Keywords: DNA nanocomposites; DNA origami nanostructure; epidermal growth factor receptor; phosphorylation; protein-DNA conjugates.

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells

2-82074 1 Kit Ask for price

Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-46091 5 x 100 ml Ask for price

Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80091 1 x 100 ml Ask for price

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-25073 5 x 100 ml Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-32074 5 x 100 ml Ask for price

Rat Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80280 1 x 100 ml Ask for price

Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80188 1 x 100 ml Ask for price

Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47091 1 Set Ask for price

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-26073 1 Set Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-33074 1 Set Ask for price

cDNA - Human Adult Normal Tissue: Skeletal Muscle

C1234171 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Skeletal Muscle

C1734171 40 reactions
EUR 385

Frozen Tissue Section - Human Adult Normal: Skeletal Muscle

T1234171 5 slides
EUR 343

Total RNA - Human Adult Normal Tissue: Skeletal Muscle

R1234171-50 50 ug
EUR 170

cDNA - Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 385

Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle

T2234171 5 slides
EUR 185

Human Adult Skeletal Muscle (Normal) Whole tissue lysate

HAL-1375 1 mg
EUR 628.8

Total Protein - Human Adult Normal Tissue: Skeletal Muscle

P1234171 1 mg
EUR 188

Total RNA - Rat Normal Tissue: Skeletal Muscle

R1434171-50 50 ug
EUR 130

Genomic DNA - Rat Normal Tissue: Skeletal Muscle

D1434171 100 ug
EUR 286

Total RNA - Mouse Normal Tissue: Skeletal Muscle

R1334171-50 50 ug
EUR 130

Membrane Protein - Human Adult Normal Tissue: Skeletal Muscle

P3234171 0.1 mg
EUR 270

Human Soft Tissue Slide (Skeletal Muscle Normal) (5 slides/pk)

HTS-12341 1 pk
EUR 343.2

Genomic DNA - Mouse Normal Tissue: Skeletal Muscle

D1334171 100 ug
EUR 286

Monkey (Cyno.) Normal Skeletal Muscle Whole tissue lysate

MCL-1197 1 mg
EUR 628.8

cDNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle

C1534171 40 reactions
EUR 385

Total Protein - Mouse Normal Tissue: Skeletal Muscle

P1334171 1 mg
EUR 188

Monkey (Rhesus) Normal Skeletal Muscle Whole tissue lysate

MRL-1247 1 mg
EUR 628.8

cDNA - Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle

C1534171-Cy 40 reactions
EUR 385

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Skeletal Muscle

R1234171-P 50 ug
EUR 370

Total RNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle

R1534171-50 50 ug
EUR 191

Normal Human Skeletal muscle (single section per slide) (5 slides/pack)

HuFPT075 5 slides
EUR 150

Total RNA - Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle

R1534171-Cy 50 ug
EUR 191

Rat Normal Skeletal muscle (single section per slide) (5 slides/pack)

RaFPT066 5 slides
EUR 150

Genomic DNA - Human Adult Normal Tissue: Skeletal Muscle, from a single donor

D1234171 100 ug
EUR 286

Mouse Normal Skeletal muscle (single section per slide) (5 slides/pack)

MoFPT071 5 slides
EUR 150

Normal Rhesus Skeletal Muscle (single section per slide) (5 slides/pack)

RsFPT066 5 slides
EUR 150

Immortalized Chicken Skeletal Muscle Cells - SV40

T9007 1x106 cells / 1.0 ml Ask for price

Skeletal Muscle Lysate

1467 0.1 mg
EUR 229.2
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skeletal Muscle Lysate

21-107 0.1 mg
EUR 342.6
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Skeletal Muscle Lysate

21-197 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

cDNA - Human Tumor Tissue: Skeletal Muscle

C1235171-10 10 reactions
EUR 274

Human Troponin C, skeletal muscle (TNNC2)

1-CSB-EP024010HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Troponin C, skeletal muscle(TNNC2) expressed in E.coli

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