Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production.
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- Gas chromatography-mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions).
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- In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4.
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- After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 μg/g) than the sonication assay (120 μg/g), both giving far higher yields than the prick assay (19 μg/g).
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- Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR).
Hairy roots from cocultivation (six months-old) accumulated as much as 1086 μg/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varied with growth kinetics, being higher at the stationary phase.
This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating the potential for a future biotechnological production of chromenes.
Keywords:Â Agrobacterium rhizogenes A4; Helianthella quinquenervis; chromenes; encecalin; medicinal plant; secondary metabolites; sonication.
Alpha-bungarotoxin, CF405s |
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| 00002-100ug | Biotium | 100uG | EUR 193.2 |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF680r |
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| 9-00003 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin, CF680r |
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| 9-00003 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF640r |
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| 9-00004 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin, CF640r |
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| 9-00004 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF488a |
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| 9-00005 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin CF488a |
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| 9-00005 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF568 |
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| 9-00006 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin, CF568 |
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| 9-00006 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF594 |
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| 9-00007 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin CF594 |
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| 9-00007 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF633 |
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| 9-00009 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin, CF633 |
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| 9-00009 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin |
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| 00010-1 | Biotium | 1MG | EUR 201.6 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Fluorescein-Alpha-bungarotoxin |
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| 00011 | Biotium | 500uG | EUR 440.4 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Tetramethylrhodamine-Alpha-bungarotoxin |
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| 00012 | Biotium | 500uG | EUR 459.6 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Fluorescein-alpha-bungarotoxin: (10x50ug) |
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| 00013 | Biotium | 10ST | EUR 500.4 |
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Description: Minimum order quantity: 1 unit of 10ST |
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Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug) |
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| 00014 | Biotium | 10ST | EUR 559.2 |
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Description: Minimum order quantity: 1 unit of 10ST |
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Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug) |
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| 00015 | Biotium | 500uG | EUR 559.2 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug) |
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| 00016 | Biotium | 10ST | EUR 627.6 |
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Description: Minimum order quantity: 1 unit of 10ST |
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Biotin-xx-a-bungarotoxin |
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| 00017 | Biotium | 500uG | EUR 519.6 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-Bungarotoxin, CF555 conjugate |
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| 9-00018 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-Bungarotoxin, CF555 conjugate |
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| 9-00018 | Biotium |
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Description: Minimum order quantity: 1 unit of 100ug |
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Biotin-cAMP, diisopropylethylammonium salt: |
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| 00020 | Biotium | 1MG | EUR 376.8 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Biotin-cAMP, diisopropylethylammonium salt: (20x50ug) |
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| 00020-1 | Biotium | 20ST | EUR 501.6 |
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Description: Minimum order quantity: 1 unit of 20ST |
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Biotin-cGMP, diisopropylethylammonium salt: |
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| 00021 | Biotium | 1MG | EUR 412.8 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Biotin-cGMP, diisopropylethylammonium salt: (20x50ug) |
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| 00021-1 | Biotium | 20ST | EUR 537.6 |
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Description: Minimum order quantity: 1 unit of 20ST |
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Cyanine 644-cAMP: |
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| 00022 | Biotium | 1MG | EUR 589.2 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Cyanine 644-cAMP: (20x50ug) |
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| 00022-1 | Biotium | 20ST | EUR 750 |
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Description: Minimum order quantity: 1 unit of 20ST |
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Fluorescein Methotrexate, triammonium salt: |
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| 00023 | Biotium | 1MG | EUR 337.2 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Staurosporine |
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| 00025 | Biotium | 100uG | EUR 166.8 |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF543 |
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| 00026 | Biotium | 500uG | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Rhodamine phalloidin 300u |
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| 00027 | Biotium | 300 | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300 |
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Biotin-xx-phalloidin |
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| 00028 | Biotium | 100U | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 100U |
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Fluorescein-phalloidin |
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| 00030 | Biotium | 300U | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300U |
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Rhodamine 110 phalloidin |
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| 00032 | Biotium | 300ST | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300ST |
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Intrinsic Type 1 Interferon (IFN1) Profile of Uncultured Human Bone Marrow CD45 low CD271 + Multipotential Stromal Cells (BM-MSCs): The Impact of Donor Age, Culture Expansion and IFNα and IFNβ Stimulation
Skeletal aging is associated with reduced proliferative potential of bone marrow (BM) multipotential stromal cells (MSCs). Recent data suggest the involvement of type 1 interferon (IFN1) signalling in hematopoietic stem cell (HSC) senescence.
Considering that BM-HSCs and BM-MSCs share the same BM niche, we investigated IFN1 expression profile in human BM-MSCs in relation to donor age, culture-expansion and IFN1 (α and β) stimulation.
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- Fluorescence-activated cell sorting was used to purify uncultured BM-MSCs from younger (19-41, n = 6) and older (59-89, n = 6) donors based on the CD45lowCD271+ phenotype, and hematopoietic-lineage cells (BM-HLCs, CD45+CD271–) were used as controls.
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- Gene expression was analysed using integrated circuits arrays in sorted fractions as well as cultured/stimulated BM-MSCs and Y201/Y202 immortalised cell lines.
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- IFN1 stimulation led to BM-MSC growth arrest and upregulation of many IFN1-stimulated genes (ISGs), with IFNβ demonstrating stronger effects. Uncultured MSCs were characterised by a moderate-level ISG expression similar to Y201 cells.
Age-related changes in ISG expression were negligible in BM-MSCs compared to BM-HLCs, and intracellular reactive oxygen species (ROS) levels in BM-MSCs did not significantly correlate with donor age. Antiaging genes Klotho and SIRT6 correlated with more ISGs in BM-MSCs than in BM-HLCs.
In patients with osteoarthritis (OA), BM-MSCs expressed considerably lower levels of several ISGs, indicating that their IFN1 signature is affected in a pathological condition. In summary, BM-MSCs possess homeostatic IFN1 gene expression signature in health, which is sensitive to in vitro culture and external IFN1 stimulation. IFN signalling may facilitate in vivo BM-MSC responses to DNA damage and combating senescence and aberrant immune activation.
Keywords:Â aging; bone marrow; mesenchymal stromal cells; senescence; type 1 interferon.
Ozone disinfection kinetics of poliovirus 1 determined by cell culture assay, RT-qPCR, and Ethidium Monoazide qPCR Reduction in a Continuous Quench-Flow Reactor
Aims:Â A continuous quench-flow (CQF) reactor was developed to collect samples at the reaction times of less than one second. The reactor is applied to determine ozone disinfection kinetics of poliovirus and to study whether EMA-qPCR can assess the viral infectivity after ozone disinfection.
Methods:Â Ozone disinfection of poliovirus was conducted in the developed CQF, and the disinfection kinetics were tested in the range of 0.7 – 5.0 seconds at ozone concentration of 0.08 and 0.25 mg/L. Inactivation, damage on viral genome and damage on capsid integrity were determined by plaque assay, quantitative reverse transcription polymerase chain reaction (RT-qPCR), and ethidium monoazide treatment coupled with RT-qPCR (EMA-qPCR), respectively.
Postsynthetic Functionalization of DNA-Nanocomposites with Proteins Yields Bioinstructive Matrices for Cell Culture Applications
We report on the directed postsynthetic functionalization of soft DNA nanocomposite materials with proteins. Using the example of the functionalization of silica nanoparticle-modified DNA polymer materials with agonists or antagonists of the epidermal growth factor receptor EGFR cell membrane receptor, we demonstrate that hierarchically structured interfaces to living cells can be established.
Due to the modular design principle, even complex DNA nanostructures can also be integrated into the materials, thereby enabling the high-precision arrangement of ligands on the lower nanometer length scale. We believe that such complex biohybrid material systems can be used for new applications in biotechnology.
Keywords:Â DNA nanocomposites; DNA origami nanostructure; epidermal growth factor receptor; phosphorylation; protein-DNA conjugates.
Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells |
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| 2-82573 | CHI Scientific | 1 Kit | Ask for price |
Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium |
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| 9-46091 | CHI Scientific | 5 x 100 ml | Ask for price |
Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium |
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| 9-25073 | CHI Scientific | 5 x 100 ml | Ask for price |
Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium |
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| 9-32074 | CHI Scientific | 5 x 100 ml | Ask for price |
Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells |
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| 9-80091 | CHI Scientific | 1 x 100 ml | Ask for price |
Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells |
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| 9-80188 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells |
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| 9-80280 | CHI Scientific | 1 x 100 ml | Ask for price |
Human Muscle PrimaCell2: Normal Smooth Muscle Cells |
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| 2-96092 | CHI Scientific | 1 Kit | Ask for price |
Mouse Muscle PrimaCell2: Normal Smooth Muscle Cells |
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| 2-82075 | CHI Scientific | 1 Kit | Ask for price |
Rat Muscle PrimaCell2: Normal Smooth Muscle Cells |
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| 2-82574 | CHI Scientific | 1 Kit | Ask for price |
Immortalized Chicken Skeletal Muscle Cells - SV40 |
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| T9007 | ABM | 1x106 cells / 1.0 ml | Ask for price |
Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
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| 9-47091 | CHI Scientific | 1 Set | Ask for price |
Human Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium |
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| 9-46092 | CHI Scientific | 5 x 100 ml | Ask for price |
cDNA from Human Adult Normal Tissue: Skeletal Muscle |
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| C1234171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Human Adult Skeletal Muscle (Normal) Whole tissue lysate |
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| HAL-1375 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Frozen Tissue Section - Human Adult Normal: Skeletal Muscle |
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| T1234171 | Biochain | 5 slides | EUR 418.8 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle |
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| T2234171 | Biochain | 5 slides | EUR 253.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
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| 9-26073 | CHI Scientific | 1 Set | Ask for price |
Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium) |
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| 9-33074 | CHI Scientific | 1 Set | Ask for price |
cDNA from Mouse Normal Tissue: Skeletal Muscle |
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| C1334171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Rat Normal Tissue: Skeletal Muscle |
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| C1434171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dog Normal Tissue: Skeletal Muscle |
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| C1734171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Soft Tissue Tissue Slide (Skeletal Muscle-Normal) |
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| 12-341-10um | ProSci | 10 um | EUR 241.8 |
Soft Tissue Tissue Slide (Skeletal Muscle-Normal) |
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| 12-341-4um | ProSci | 4 um | EUR 216.6 |
Human Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells |
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| 9-80092 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium |
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| 9-25074 | CHI Scientific | 5 x 100 ml | Ask for price |
Mouse Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium |
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| 9-32075 | CHI Scientific | 5 x 100 ml | Ask for price |
Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells |
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| 2-96009 | CHI Scientific | 1 Kit | Ask for price |
Human Cervix PrimaCell3: Normal Cervical Smooth Muscle Cells |
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| 2-96046 | CHI Scientific | 1 Kit | Ask for price |
Human Esophagus PrimaCell2: Normal Esophageal Smooth Muscle Cells |
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| 2-96056 | CHI Scientific | 1 Kit | Ask for price |
Human Heart PrimaCell1: Normal Aortic Smooth Muscle Cells |
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| 2-96066 | CHI Scientific | 1 Kit | Ask for price |
Human Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells |
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| 2-96072 | CHI Scientific | 1 Kit | Ask for price |
Human Rectal PrimaCell: Normal Rectal Smooth Muscle Cells |
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| 2-96100 | CHI Scientific | 1 Kit | Ask for price |
Rat Bladder PrimaCell3: Normal Smooth Muscle Cells |
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| 2-82508 | CHI Scientific | 1 Kit | Ask for price |
Total Protein from Human Adult Normal Tissue: Skeletal Muscle |
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| P1234171 | Biochain | 1 mg | EUR 256.8 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Membrane Protein from Human Adult Normal Tissue: Skeletal Muscle |
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| P3234171 | Biochain | 0.1 mg | EUR 342 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Total RNA from Human Adult Normal Tissue: Skeletal Muscle |
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| R1234171-50 | Biochain | 50 ug | EUR 213.6 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Skeletal Muscle |
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| C1534171 | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Skeletal Muscle |
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| C1534171-Cy | Biochain | 40 reactions | EUR 451.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA from Mouse Normal Tissue: Skeletal Muscle |
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| D1334171 | Biochain | 100 ug | EUR 338.4 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA from Rat Normal Tissue: Skeletal Muscle |
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| D1434171 | Biochain | 100 ug | EUR 338.4 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Total Protein from Mouse Normal Tissue: Skeletal Muscle |
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| P1334171 | Biochain | 1 mg | EUR 256.8 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Monkey (Cyno.) Normal Skeletal Muscle Whole tissue lysate |
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| MCL-1197 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Monkey (Rhesus) Normal Skeletal Muscle Whole tissue lysate |
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| MRL-1247 | Alpha Diagnostics | 1 mg | EUR 628.8 |
