Systembio Distributor

Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

Lentivectors

Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production.

 

    • Gas chromatography-mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions).

 

    • In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4.

 

    • After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 μg/g) than the sonication assay (120 μg/g), both giving far higher yields than the prick assay (19 μg/g).

 

    • Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR).

 

Hairy roots from cocultivation (six months-old) accumulated as much as 1086 μg/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varied with growth kinetics, being higher at the stationary phase.

 

This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating the potential for a future biotechnological production of chromenes.

 

Lentivectors
Lentivectors
Keywords: Agrobacterium rhizogenes A4; Helianthella quinquenervis; chromenes; encecalin; medicinal plant; secondary metabolites; sonication.

 

Tapered Micro Tips

0-120-0007 3 mm
EUR 344

Tapered Micro Tips 5 mm Diameter Tapered Titanium Micro Tip

0-120-0008 5 mm
EUR 358

10 mm Diameter Solid Titanium Tip

0-120-0009 10 mm
EUR 504

13 mm Diameter Tapped Titanium Tip

0-120-0010 13 mm
EUR 595

13 mm Diameter Solid Titanium Tip

0-120-0011 13 mm
EUR 533

19 mm Diameter Tapped Titanium Tip

0-120-0012 19 mm
EUR 639

19 mm Diameter Solid Titanium Tip

0-120-0013 19 mm
EUR 570

25 mm Diameter Tapped Titanium Tip

0-120-0014 25 mm
EUR 682

25 mm Diameter Solid Titanium Tip

0-120-0015 25 mm
EUR 614

Flat Replaceable Tips 13 mm Diameter Flat Titanium Tip

0-120-0016 13 mm
EUR 86
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip

0-120-0017 19 mm
EUR 92
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip

0-120-0018 25 mm
EUR 100
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Titanium Cup Tip, 250 ml

0-120-0019 250 ml
EUR 1377
Description: includes Interface Washers

Microtube Tray, 8 Position (for 250 ml Cup Tip)

0-120-0021 each
EUR 113
Description: includes Interface Washers

Continuous Flow Chamber

0-120-0026 each
EUR 774
Description: includes Interface Washers

Interface Washers 1.5” Diameter, 5/Pkg

0-120-003 1.5'' Diameter
EUR 31

13 mm Diameter Solid Titanium Extender Tip

0-120-0032 13 mm
EUR 301

13 mm Diameter Tapped Titanium Extender Tip

0-120-0033 13 mm
EUR 362

19 mm Diameter Solid Titanium Extender Tip

0-120-0034 19 mm
EUR 315

19 mm Diameter Tapped Titanium Extender Tip

0-120-0035 19 mm
EUR 375

25 mm Diameter Solid Titanium Extender Tip

0-120-0036 25 mm
EUR 328

25 mm Diameter Tapped Titanium Extender Tip

0-120-0037 25 mm
EUR 389

KoldPod, 1.5ml Micro Tube

0-120-0038 1.5 ml
EUR 152
Description: Thermo conductive tube pods

KoldPod, 15ml Conical Tube

0-120-0039 15 ml
EUR 275
Description: Thermo conductive tube pods

KoldPod, 50ml Conical Tube

0-120-0040 50 ml
EUR 290
Description: Thermo conductive tube pods

Model 150 V/T Ultrasonic Homogenizer

0-121-0002 210-240V/50-60Hz
EUR 2920
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.

Model 300 V/T Ultrasonic Homogenizer

0-122-0002 210-240V/50-60Hz
EUR 3520
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample.

SONABOZ Sound Abating Chamber

0-125-0001 each
EUR 1020
Description: Reduces cavitational sound emitted during processing.

Model 3000 Ultrasonic Homogenizer

0-127-0002 210-240V/50-60Hz
EUR 4120
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample.

Model 3000MP Ultrasonic Homogenizer

0-128-0002 210-240V/50-60Hz
EUR 4720
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.

OMNICON® Zone Reader, 210-240V/50-60Hz

0-131-0002 210-240V/50-60Hz
EUR 35200
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.

OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz

0-134-0002 210-240V/50-60Hz
EUR 32200
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.

Peni Cylinder Dispenser with Manual Hopper

0-144-0002 each
EUR 5406
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish.

Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz

0-144-0003 100-240V/50-60Hz
EUR 6254
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection.

Stainless Steel Peni Cylinder with Flat Face

0-144-0005 6mm I.D. x 8mm O.D. x 10mm Long
EUR 399
Description: sold in packages of 100 pieces

Stainless Steel Peni Cylinder with Chamfered Face

0-144-0006 6mm I.D. x 8mm O.D. x 10mm Long
EUR 412
Description: sold in packages of 100 pieces

Intrinsic Type 1 Interferon (IFN1) Profile of Uncultured Human Bone Marrow CD45 low CD271 + Multipotential Stromal Cells (BM-MSCs): The Impact of Donor Age, Culture Expansion and IFNα and IFNβ Stimulation

 

Skeletal aging is associated with reduced proliferative potential of bone marrow (BM) multipotential stromal cells (MSCs). Recent data suggest the involvement of type 1 interferon (IFN1) signalling in hematopoietic stem cell (HSC) senescence.

 

Considering that BM-HSCs and BM-MSCs share the same BM niche, we investigated IFN1 expression profile in human BM-MSCs in relation to donor age, culture-expansion and IFN1 (α and β) stimulation.

 

    • Fluorescence-activated cell sorting was used to purify uncultured BM-MSCs from younger (19-41, n = 6) and older (59-89, n = 6) donors based on the CD45lowCD271+ phenotype, and hematopoietic-lineage cells (BM-HLCs, CD45+CD271) were used as controls.

 

    • Gene expression was analysed using integrated circuits arrays in sorted fractions as well as cultured/stimulated BM-MSCs and Y201/Y202 immortalised cell lines.

 

    • IFN1 stimulation led to BM-MSC growth arrest and upregulation of many IFN1-stimulated genes (ISGs), with IFNβ demonstrating stronger effects. Uncultured MSCs were characterised by a moderate-level ISG expression similar to Y201 cells.

 

Age-related changes in ISG expression were negligible in BM-MSCs compared to BM-HLCs, and intracellular reactive oxygen species (ROS) levels in BM-MSCs did not significantly correlate with donor age. Antiaging genes Klotho and SIRT6 correlated with more ISGs in BM-MSCs than in BM-HLCs.

 

In patients with osteoarthritis (OA), BM-MSCs expressed considerably lower levels of several ISGs, indicating that their IFN1 signature is affected in a pathological condition. In summary, BM-MSCs possess homeostatic IFN1 gene expression signature in health, which is sensitive to in vitro culture and external IFN1 stimulation. IFN signalling may facilitate in vivo BM-MSC responses to DNA damage and combating senescence and aberrant immune activation.

 

Keywords: aging; bone marrow; mesenchymal stromal cells; senescence; type 1 interferon.

 

Ozone disinfection kinetics of poliovirus 1 determined by cell culture assay, RT-qPCR, and Ethidium Monoazide qPCR Reduction in a Continuous Quench-Flow Reactor

 

Aims: A continuous quench-flow (CQF) reactor was developed to collect samples at the reaction times of less than one second. The reactor is applied to determine ozone disinfection kinetics of poliovirus and to study whether EMA-qPCR can assess the viral infectivity after ozone disinfection.

 

Methods: Ozone disinfection of poliovirus was conducted in the developed CQF, and the disinfection kinetics were tested in the range of 0.7 – 5.0 seconds at ozone concentration of 0.08 and 0.25 mg/L. Inactivation, damage on viral genome and damage on capsid integrity were determined by plaque assay, quantitative reverse transcription polymerase chain reaction (RT-qPCR), and ethidium monoazide treatment coupled with RT-qPCR (EMA-qPCR), respectively.

 

 

Postsynthetic Functionalization of DNA-Nanocomposites with Proteins Yields Bioinstructive Matrices for Cell Culture Applications

 

We report on the directed postsynthetic functionalization of soft DNA nanocomposite materials with proteins. Using the example of the functionalization of silica nanoparticle-modified DNA polymer materials with agonists or antagonists of the epidermal growth factor receptor EGFR cell membrane receptor, we demonstrate that hierarchically structured interfaces to living cells can be established.

 

Due to the modular design principle, even complex DNA nanostructures can also be integrated into the materials, thereby enabling the high-precision arrangement of ligands on the lower nanometer length scale. We believe that such complex biohybrid material systems can be used for new applications in biotechnology.

 

Keywords: DNA nanocomposites; DNA origami nanostructure; epidermal growth factor receptor; phosphorylation; protein-DNA conjugates.

Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-46091 5 x 100 ml Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells

2-82074 1 Kit Ask for price

Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80091 1 x 100 ml Ask for price

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-25073 5 x 100 ml Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Medium

9-32074 5 x 100 ml Ask for price

Rat Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80280 1 x 100 ml Ask for price

Mouse Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells

9-80188 1 x 100 ml Ask for price

Human Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47091 1 Set Ask for price

Rat Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-26073 1 Set Ask for price

Mouse Muscle PrimaCell1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-33074 1 Set Ask for price

cDNA - Human Adult Normal Tissue: Skeletal Muscle

C1234171 40 reactions
EUR 424

Tissue, Total Protein, Human Adult Normal, Skeletal Muscle

MBS657322-1mg 1mg
EUR 565

Tissue, Total Protein, Human Adult Normal, Skeletal Muscle

MBS657322-5x1mg 5x1mg
EUR 2315

Frozen Tissue Section - Human Adult Normal: Skeletal Muscle

T1234171 5 slides
EUR 377

Tissue, Section, Human Adult Normal, Skeletal Muscle (Frozen)

MBS640631-5Sections 5Sections
EUR 635

Tissue, Section, Human Adult Normal, Skeletal Muscle (Frozen)

MBS640631-5x5Sections 5x5Sections
EUR 2640

Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle

T2234171 5 slides
EUR 204

Tissue, Section, Human Adult Normal, Skeletal Muscle (Paraffin)

MBS640137-5Sections 5Sections
EUR 460

Tissue, Section, Human Adult Normal, Skeletal Muscle (Paraffin)

MBS640137-5x5Sections 5x5Sections
EUR 1925

Tissue, Membrane Protein, Human Adult Normal, Skeletal Muscle

MBS639871-01mg 0.1mg
EUR 570

Tissue, Membrane Protein, Human Adult Normal, Skeletal Muscle

MBS639871-5x01mg 5x0.1mg
EUR 2345

Total RNA - Human Adult Normal Tissue: Skeletal Muscle

R1234171-50 50 ug
EUR 221

Human Adult Skeletal Muscle (Normal) Whole tissue lysate

HAL-1375 1 mg
EUR 628.8

Human Skeletal Muscle Cells

ABC-TC3797 1 vial Ask for price
Description: Skeletal muscle cells are one of the largest cells in the body. They are multinucleate formed by the fusion of myoblasts. Skeletal muscle regeneration is a complex process in which many factors are involved. When skeletal muscle suffers an injury, quiescent resident myoblasts called satellite cells are activated to proliferate, migrate, and finally differentiate. Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase (MAPK) have been suggested to play an important role in skeletal muscle growth. RI-stimulated glucose transport in cultured human skeletal muscle is mediated by GLUT4 and heparan sulfate proteoglycan is involved in skeletal muscle differentiation. The fusion of mononucleated cells to form multinucleated myotubes is a central event in skeletal muscle development. Controlling the onset and progression of this process is a complex set of interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful model for studying this process of cell differentiation.HSkMC from Gentaur Research Laboratories are isolated from human muscle of the pectoral girdle. HSkMC are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HSkMC are characterized by immunofluorescent method with antibodies to myosin, actin and actinin. HSkMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSkMC are guaranteed to further expand for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Skeletal Muscle Cells

CSC-C3604 One Frozen vial Ask for price

Human Skeletal Muscle Cells 

CSC-C4867L One Frozen vial Ask for price

Total Protein - Human Adult Normal Tissue: Skeletal Muscle

P1234171 1 mg
EUR 216

cDNA - Dog Normal Tissue: Skeletal Muscle

C1734171 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 424

Tissue, Total RNA, Human Adult Normal, Skeletal Muscle, BioGenomics

MBS638548-005mg 0.05mg
EUR 495

Tissue, Total RNA, Human Adult Normal, Skeletal Muscle, BioGenomics

MBS638548-5x005mg 5x0.05mg
EUR 1995

cDNA - Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 424

Membrane Protein - Human Adult Normal Tissue: Skeletal Muscle

P3234171 0.1 mg
EUR 311

Tissue, Genomic DNA, Human Adult Normal, Skeletal Muscle, BioGenomics

MBS654357-01mg 0.1mg
EUR 575

Tissue, Genomic DNA, Human Adult Normal, Skeletal Muscle, BioGenomics

MBS654357-5x01mg 5x0.1mg
EUR 2440

Human Soft Tissue Slide (Skeletal Muscle Normal) (5 slides/pk)

HTS-12341 1 pk
EUR 343.2

Total RNA - Rat Normal Tissue: Skeletal Muscle

R1434171-50 50 ug
EUR 156

Genomic DNA - Rat Normal Tissue: Skeletal Muscle

D1434171 100 ug
EUR 329

Total RNA - Mouse Normal Tissue: Skeletal Muscle

R1334171-50 50 ug
EUR 156

Human Skeletal Muscle Cells, Foetal

ABC-TC3801 1 vial Ask for price
Description: Human Skeletal Muscle Cells (HSkMC) are isolated from the limbal skeletal muscle. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings.

Tissue cDNA, First Strand, Human Adult Normal, Skeletal Muscle, BioGenomics

MBS652565-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Skeletal Muscle, BioGenomics

MBS652565-5x40Tests 5x40Tests
EUR 2835

Human Primary Skeletal Muscle Cells

T4069 5x10^5 cells / 1.0 ml
EUR 625

Genomic DNA - Mouse Normal Tissue: Skeletal Muscle

D1334171 100 ug
EUR 329

Leave a Reply

Your email address will not be published.