A novel technique to make viscoelastic polyacrylamide gels for cell tradition and traction drive microscopy
Polyacrylamide hydrogels are generally utilized in cell biology, notably to domesticate cells on tender surfaces. Polyacrylamide gels are purely elastic and properly tailored to cell tradition as they’re inert and might be conjugated with adhesion proteins.
Right here, we report a way to make viscoelastic polyacrylamide gels with mechanical properties extra carefully resembling organic tissues and appropriate for cell tradition in vitro. We reveal that these gels can be utilized for traction drive microscopy experiments.
We additionally present that a number of cell sorts reply to the viscoelasticity of their substrate and that viscous dissipation has an affect on cell spreading, contractility, and motility. This new materials gives new alternatives for investigating how regular or malignant cells sense and reply to viscous dissipation throughout the extra-cellular matrix.
Follicular Dendritic Cells antibody |
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| 10R-6696 | Fitzgerald | 100 ug | EUR 424.8 |
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Description: Mouse monoclonal Follicular Dendritic Cells antibody |
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Follicular Dendritic Cells antibody |
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| 10R-M164A | Fitzgerald | 500 ug | EUR 846 |
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Description: Mouse monoclonal Follicular Dendritic Cells antibody |
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Immortalized Mouse Dendritic Cells (MutuDC1940) |
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| T0528 | ABM | 1x106 cells / 1.0 ml | EUR 1243.2 |
Dendritic/Interdigitating Cells Antibody |
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| abx415668-2ml | Abbexa | 2 ml | EUR 727.2 |
Immortalized Mouse Spleen Dendritic Cells (SRDC) |
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| T0140 | ABM | 1x106 cells / 1.0 ml | Ask for price |
ED5 Antigen (Follicular Dendritic Cells) Antibody |
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| abx415025-025mg | Abbexa | 0.25 mg | EUR 678 |
Mouse Anti Human Follicular Dendritic Cells Monoclonal Antibody |
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| DMABT-52181MH | Creative Diagnostics | 0.2 mg | EUR 1431.6 |
Mouse Anti Human Follicular Dendritic Cells Monoclonal Antibody |
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| DMABT-52196MH | Creative Diagnostics | 25 µg | EUR 446.4 |
Mouse Anti Human Follicular Dendritic Cells Monoclonal Antibody |
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| DMABT-52197MH | Creative Diagnostics | 0.2 mg | EUR 889.2 |
CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC942083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF594 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC942083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF594 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC802083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF680 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC802083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF680 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC702083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF770 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC702083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF770 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC882083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF488A conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC882083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF488A conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC812083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF680R conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC812083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF680R conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC052083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF405M conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC052083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF405M conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC042083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF405S conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC042083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF405S conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC402083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF640R conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC402083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF640R conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCAP2083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCAP2083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCA2083-250 | Biotium | 250uL | EUR 472.8 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), APC conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCP2083-250 | Biotium | 250uL | EUR 472.8 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), PerCP conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCR2083-250 | Biotium | 250uL | EUR 472.8 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), RPE conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCH2083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCH2083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCB2083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Biotin conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNCB2083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Biotin conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNUB2083-100 | Biotium | 100uL | EUR 316.8 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Concentration: 0.2mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNUB2083-50 | Biotium | 50uL | EUR 486 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNUB2083-500 | Biotium | 500uL | EUR 615.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), Concentration: 0.2mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC472083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF647 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC472083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF647 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC552083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF555 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC552083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF555 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC612083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF660R conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC612083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF660R conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC432083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF543 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC432083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF543 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC682083-100 | Biotium | 100uL | EUR 279.6 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF568 conjugate, Concentration: 0.1mg/mL |
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CD86 (Dendritic Cells Maturation Marker) (rC86/1146) Antibody |
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| BNC682083-500 | Biotium | 500uL | EUR 654 |
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Description: Primary antibody against CD86 (Dendritic Cells Maturation Marker) (rC86/1146), CF568 conjugate, Concentration: 0.1mg/mL |
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Mouse Anti Rat Follicular Dendritic Cells Monoclonal Antibody |
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| DMABT-49803MR | Creative Diagnostics | 0.25 mg | EUR 920.4 |
Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody |
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| M00220 | BosterBio | 100ug/vial | EUR 476.4 |
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Description: Mouse Monoclonal CD86 (Dendritic Cells Maturation Marker) Antibody. Validated in Flow Cytometry, IP and tested in Human, Mouse, Rat. |
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Monoclonal CD86 (Dendritic Cells Maturation Marker) Antibody, Clone: BU63 |
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| APR10944G | Leading Biology | 7 ml | EUR 580.8 |
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Description: A Monoclonal antibody against Human CD86 (Dendritic Cells Maturation Marker). The antibodies are raised in Mouse and are from clone BU63. This antibody is applicable in WB, IHC and IF, FC, IP |
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Rat Anti Mouse Dendritic/Interdig. Cells Monoclonal Antibody |
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| DMABT-54517RM | Creative Diagnostics | 2 ml | EUR 920.4 |
CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC941781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF594 conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC941781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF594 conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC801781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF680 conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC801781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF680 conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC701781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF770 conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC701781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF770 conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC881781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF488A conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC881781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF488A conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC811781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF680R conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC811781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF680R conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC051781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF405M conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC051781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF405M conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC041781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF405S conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC041781-500 | Biotium | 500uL | EUR 652.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF405S conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC401781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF640R conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC401781-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF640R conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNCAP1781-100 | Biotium | 100uL | EUR 238.8 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNCAP1781-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNCA1781-250 | Biotium | 250uL | EUR 459.6 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),APC conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNCP1781-250 | Biotium | 250uL | EUR 459.6 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),PerCP conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNCR1781-250 | Biotium | 250uL | EUR 459.6 |
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Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),RPE conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNCH1781-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
|||
| BNCH1781-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
|||
CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
|||
| BNCB1781-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),Biotin conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
|||
| BNCB1781-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),Biotin conjugate, Concentration: 0.1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNUB1781-100 | Biotium | 100uL | EUR 250.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781), Concentration: 0.2mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
|||
| BNUB1781-500 | Biotium | 500uL | EUR 549.6 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781), Concentration: 0.2mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNUM1781-50 | Biotium | 50uL | EUR 474 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781), 1mg/mL |
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CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
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| BNC471781-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF647 conjugate, Concentration: 0.1mg/mL |
|||
CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
|||
| BNC471781-500 | Biotium | 500uL | EUR 652.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF647 conjugate, Concentration: 0.1mg/mL |
|||
CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781) Antibody |
|||
| BNC611781-100 | Biotium | 100uL | EUR 238.8 |
|
Description: Primary antibody against CD209 / DC-SIGN (Pathogen Receptor on Dendritic Cells) (C209/1781),CF660R conjugate, Concentration: 0.1mg/mL |
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Homogenization of preliminary cell distribution by secondary move of medium improves cell tradition effectivity
Homogenization of the preliminary cell distribution is important for efficient cell improvement. Nevertheless, there are few earlier experiences on environment friendly cell seeding strategies, regardless that the preliminary cell distribution has a big impact on cell proliferation.
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- Dense cell areas have an inverse influence on cell improvement, often known as contact inhibition. On this examine, we developed a way to homogenize the cell seeding density utilizing secondary move, or Ekman transportation, induced by orbital motion of the tradition dish.
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- We developed an orbital shaker machine that may stir the medium in a 35-mm tradition dish by shaking the dish alongside a round orbit with 2 mm of eccentricity.
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- The distribution of cells within the tradition dish might be managed by the rotational pace of the orbital shaker, enabling dispersion of the preliminary cell distribution.
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- The experimental outcomes indicated that the cell density turned most homogeneous at 61 rpm. We additional evaluated the cell proliferation after homogenization of the preliminary cell density at 61 rpm.
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- The outcomes revealed 36% greater proliferation for the stirred samples in contrast with the non-stirred management samples.
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- The current findings point out that homogenization of the preliminary cell density by Ekman transportation contributes to the achievement of upper cell proliferation.
Quantitative Picture-Primarily based Cell Viability (QuantICV) Assay for Microfluidic 3D Tissue Tradition Purposes
Microfluidic 3D tissue tradition programs are engaging for in vitro drug testing purposes as a result of capability of those platforms to generate 3D tissue fashions and carry out drug testing at a really small scale.
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- Nevertheless, the minute cell quantity and liquid quantity impose important technical challenges to carry out quantitative cell viability measurements utilizing typical colorimetric or fluorometric assays, reminiscent of MTS or Alamar Blue.
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- Equally, live-dead staining approaches usually make the most of metabolic dyes that sometimes label the cytoplasm of reside cells, which makes it troublesome to phase and depend particular person cells in compact 3D tissue cultures.
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- On this paper, we current a quantitative image-based cell viability (QuantICV) assay approach that circumvents present challenges of performing the quantitative cell viability assay in microfluidic 3D tissue cultures.
A pair of cell-impermeant nuclear dyes (EthD-1 and DAPI) had been used to sequentially label the nuclei of necrotic and complete cell populations, respectively. Confocal microscopy and picture processing algorithms had been employed to visualise and quantify the cell nuclei within the 3D tissue quantity.
The QuantICV assay was validated and confirmed good concordance with the standard bulk MTS assay in static 2D and 3D tumor cell cultures. Lastly, the QuantICV assay was employed as an on-chip readout to find out the differential dose responses of parental and metastatic 3D oral squamous cell carcinoma (OSCC) to Gefitinib in a microfluidic 3D tradition machine.
This proposed approach might be helpful in microfluidic cell cultures in addition to in a scenario the place typical cell viability assays are usually not accessible.
Human T helper 17 cell (Th17) ELISA Kit |
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| YLA3873HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human T helper 17 cell (Th17) ELISA Kit |
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| YLA3873HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Human T helper 22 cell (Th22) ELISA Kit |
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| YLA3874HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human T helper 22 cell (Th22) ELISA Kit |
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| YLA3874HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Dog T helper 17 cell ELISA kit |
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| E08T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Canine T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog T helper 17 cell ELISA kit |
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| E08T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Canine T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog T helper 17 cell ELISA kit |
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| E08T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Canine T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat T helper 17 cell ELISA kit |
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| E02T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rat T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat T helper 17 cell ELISA kit |
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| E02T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rat T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat T helper 17 cell ELISA kit |
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| E02T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rat T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig T helper 17 cell ELISA kit |
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| E07T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Porcine T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig T helper 17 cell ELISA kit |
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| E07T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Porcine T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig T helper 17 cell ELISA kit |
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| E07T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Porcine T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat T helper 17 cell ELISA kit |
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| E06T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Goat T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat T helper 17 cell ELISA kit |
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| E06T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Goat T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat T helper 17 cell ELISA kit |
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| E06T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Goat T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse T helper 17 cell ELISA kit |
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| E03T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Mouse T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse T helper 17 cell ELISA kit |
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| E03T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Mouse T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse T helper 17 cell ELISA kit |
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| E03T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Mouse T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit T helper 17 cell ELISA kit |
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| E04T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit T helper 17 cell ELISA kit |
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| E04T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit T helper 17 cell ELISA kit |
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| E04T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey T helper 17 cell ELISA kit |
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| E09T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Monkey T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey T helper 17 cell ELISA kit |
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| E09T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Monkey T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey T helper 17 cell ELISA kit |
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| E09T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Monkey T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig T helper 17 cell ELISA kit |
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| E05T0053-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig T helper 17 cell ELISA kit |
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| E05T0053-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig T helper 17 cell ELISA kit |
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| E05T0053-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig T helper 17 cell in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human T Helper 1 (TH1)ELISA Kit |
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| YLA4223HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human T Helper 1 (TH1)ELISA Kit |
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| YLA4223HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Human T Helper 2(TH2) ELISA Kit |
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| YLA4239HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human T Helper 2(TH2) ELISA Kit |
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| YLA4239HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
rAAV2-retro helper |
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| PVT15945 | Lifescience Market | 2 ug | EUR 390 |
TET 830 modified/T – helper epitope from tetanus toxoid |
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| 5-02004 | CHI Scientific | 4 x 1mg | Ask for price |
CD4+ T cells, Negatively Selected (Human) |
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| 79752 | BPS Bioscience | 10 million cells | EUR 395 |
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Description: Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate._x000D_Source Normal human PBMC from Leukapheresis Sample |
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AAV-1 Helper Free Packaging System |
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| VPK-401 | Cell Biolabs | 1 kit | EUR 1166.4 |
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Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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AAV-2 Helper Free Packaging System |
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| VPK-402 | Cell Biolabs | 1 kit | EUR 1166.4 |
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Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
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