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Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease

Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease

Three-dimensional (3D) cell tradition strategies have been broadly used on a variety of cell varieties, together with stem cells to modulate exactly the mobile biophysical and biochemical microenvironment and management varied cell signaling cues. Consequently, extra in vivo-like microenvironments are recapitulated, notably by means of the formation of multicellular spheroids and organoids, which can yield extra legitimate mechanisms of illness.

 

    • Lately, genome-engineering instruments akin to CRISPR Cas9 have expanded the repertoire of methods to regulate gene expression, which enhances exterior signaling cues with intracellular management parts.

 

 

  • Consequently, the mix of CRISPR Cas9 and 3D cell tradition strategies improve our understanding of the molecular mechanisms underpinning a number of illness phenotypes and should result in creating new therapeutics which will advance extra rapidly and successfully into medical candidates.

 

  • As well as, utilizing CRISPR Cas9 instruments to rescue genes brings us one step nearer to its use as a gene remedy instrument for varied degenerative illnesses.

 

  • Herein, we offer an summary of bridging of CRISPR Cas9 genome enhancing with 3D spheroid and organoid cell tradition to higher perceive illness development in each affected person and non-patient derived cells, and we tackle potential remaining gaps that should be overcome to achieve widespread use.
Lentivectors
Lentivectors
Key phrases: 3D cell tradition microsystem; microscale 3D printing; organoids; affected person derived cells (PDCs); pluripotent stem cell.

 

Bone Marrow-Derived Dendritic Cell Cultures from RAG -/- Mice Embrace IFN-γ-Producing NK Cells

 

Dendritic cells (DCs) play a key position within the initiation of an immune response and are generally known as “skilled” APCs due to their skill to activate naive T cells. A broadly used technique to generate DCs in vitro is to tradition bone marrow (BM) cells or blood monocytes within the presence of GM-CSF and IL-4.

 

On this research, we present {that a} small inhabitants of NK cells residing within the BM of RAG-/-, however not RAG-/- γc chain-/- mice, stay within the DC tradition and is the supply of IFN-γ produced after stimulation with LPS. These cells, which can signify early promoters of LPS-induced responses, must be taken into consideration when decoding experiments utilizing BM-derived DCs.

 

Human Primary Nasal Epithelial Cells

T4014 5x10^5 cells / 1.0 ml
EUR 1375

Immortalized Human Nasal Epithelial Cells - SV40

T9243 1x10^6 cells / 1.0 ml
EUR 2750

Immortalized Human Nasal Epithelial Cells - SV40

T0440 1x106 cells / 1.0 ml
EUR 2750

Immortalized Human Nasal Epithelial Cells-SV40

CSC-I9157L One Frozen vial Ask for price

Normal Human Small Airway Epithelial Cells

T5514 5x10^5 cells / 1.0 ml
EUR 875

Immortalized Human Nasal Epithelial Cells - SV40 & hTERT

T9245 1x10^6 cells / 1.0 ml Ask for price

Normal Human Primary Broncial Epithelial Cells

T5511 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Tracheal Epithelial Cells

T5512 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Tracheal Epithelial Cells

T5513 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Bronchial Epithelial Cells

T5510 5x10^5 cells / 1.0 ml
EUR 875

Human Eye PrimaCell4: Normal Lens Epithelial Cells

2-96060 1 Kit Ask for price

Normal Human Primary Small Airway Epithelial Cells

T5515 5x10^5 cells / 1.0 ml
EUR 875

Human Eye PrimaCell2: Normal Corneal Epithelial Cells

2-96058 1 Kit Ask for price

Human Airway PrimaCell1: Normal Airway Epithelial Cells

2-96001 1 Kit Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-96075 1 Kit Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-96094 1 Kit Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells

2-96112 1 Kit Ask for price

Normal Human Primary Intestinal Epithelial Cells Ilium

T5528 5x10^5 cells / 1.0 ml
EUR 875

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-96007 1 Kit Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-96044 1 Kit Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells

2-96115 1 Kit Ask for price

Normal Human Primary Intestinal Epithelial Cells Jejunum

T5527 5x10^5 cells / 1.0 ml
EUR 875

Human Prostate PrimaCell: Normal Prostate Epithelial Cells

2-96099 1 Kit Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells

2-96113 1 Kit Ask for price

Normal Human Primary Intestinal Epithelial Cells Duodenem

T5526 5x10^5 cells / 1.0 ml
EUR 875

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-96047 1 Kit Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-96055 1 Kit Ask for price

Human Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-96061 1 Kit Ask for price

Human Kidney PrimaCell7: Normal Renal Tubular Epithelial Cells

2-96080 1 Kit Ask for price

Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-96104 1 Kit Ask for price

Normal Human Primary Intestinal Epithelial Cells Ascending Colon

T5523 5x10^5 cells / 1.0 ml
EUR 975

Normal Human Primary Intestinal Epithelial Cells Transverse Colon

T5524 5x10^5 cells / 1.0 ml
EUR 975

Normal Human Primary Intestinal Epithelial Cells Descending Colon

T5525 5x10^5 cells / 1.0 ml
EUR 975

Human Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium

9-46060 5 x 100 ml Ask for price

Immortalized Porcine Nasal Mucosal Epithelial Cells

CSC-I2069Z One Frozen vial Ask for price

Human Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells

2-96071 1 Kit Ask for price

Rat Eye PrimaCell4: Normal Lens Epithelial Cells

2-82541 1 Kit Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium

9-46058 5 x 100 ml Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-96023 1 Kit Ask for price

Mouse Eye PrimaCell4: Normal Lens Epithelial Cells

2-82040 1 Kit Ask for price

Normal Human Primary Intrahepatic Biliary Epithelial Cells (Cholagioctyes)

T5555 1x10^6 cells / 1.0 ml
EUR 1085

Human Airway PrimaCell1: Normal Airway Epithelial Cells Growth Medium

9-46001 5 x 100 ml Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-46075 5 x 100 ml Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium

9-46094 5 x 100 ml Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells Growth Medium

9-46112 5 x 100 ml Ask for price

Human Thyroid PrimaCell: Normal Thyroid Epithelial Cells Growth Medium

9-46124 5 x 100 ml Ask for price

NHBE (Normal Human Bronchial Epithelial Cells) w/ Retinoic Acid, BEGM

LOCC-2540 each
EUR 1861.75

Rat Eye PrimaCell2: Normal Corneal Epithelial Cells

2-82539 1 Kit Ask for price

Human Eye Tissue Preparation Buffer 4: Normal Lens Epithelial Cells

9-80060 1 x 100 ml Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82588 1 Kit Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-46007 5 x 100 ml Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium

9-46044 5 x 100 ml Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium

9-46099 5 x 100 ml Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells Growth Medium

9-46113 5 x 100 ml Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells Growth Medium

9-46115 5 x 100 ml Ask for price

Human Thyroid Tissue Preparation Buffer: Normal Thyroid Epithelial Cells

9-80317 1 x 100 ml Ask for price

Human Airway PrimaCell2: Normal Bronchial and Tracheal Epithelial Cells

2-96002 1 Kit Ask for price

Mouse Eye PrimaCell2: Normal Corneal Epithelial Cells

2-82038 1 Kit Ask for price

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

Rat Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-82576 1 Kit Ask for price

Human Eye Tissue Preparation Buffer 2: Normal Corneal Epithelial Cells

9-80058 1 x 100 ml Ask for price

Mouse Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82092 1 Kit Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium

9-46047 5 x 100 ml Ask for price

Human Prostate Tissue Preparation Buffer: Normal Prostate Epithelial Cells

9-80099 1 x 100 ml Ask for price

Human Urethral Tissue Preparation Buffer: Normal Urethral Epithelial Cells

9-80113 1 x 100 ml Ask for price

Human Colon Tissue Preparation Buffer: Normal Colorectal Epithelial Cells

9-80297 1 x 100 ml Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82057 1 Kit Ask for price

Mouse Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-82077 1 Kit Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82506 1 Kit Ask for price

Rat Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-82524 1 Kit Ask for price

Human Airway Tissue Preparation Buffer 1: Normal Airway Epithelial Cells

9-80001 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Human Ovary Tissue Preparation Buffer 1: Normal Ovarian Epithelial Cells

9-80094 1 x 100 ml Ask for price

Human Ureter Tissue Preparation Buffer 2: Normal Ureter Epithelial Cells

9-80112 1 x 100 ml Ask for price

 

Impact of Elasticity on Electrospun Styrene-Butadiene-Styrene Fibrous Membrane Cell Tradition Behaviors

 

On this research, elastic styrene-butadiene-styrene (SBS), non-elastic SBS and their blends at totally different ratios have been electrospun into fibrous membranes and their cell biocompatibility was evaluated. The as-spun fibers confirmed a mean fiber diameter of two µm, and the fibrous membranes had pore measurement of 8 ± 0.01 µm.

 

The mixing ratios of the elastic with non-elastic SBSs confirmed little impact on fibrous construction, however affected the mechanical properties. All SBS membrane confirmed no cytotoxicity on endothelial cells (ECs). ECs connected and proliferated on all of the SBS fibrous membrane scaffolds no matter their elasticity.

 

ECs maintained their polygonal form on the scaffolds and so they tended to orient alongside the fiber size. The SBS fibrous samples with elastic: non-elastic SBS weight ratios of 1:1 and a couple of:three showed higher cell viability than that of elastic and non-elastic SBS.

 

Tradition and software of conditionally reprogrammed major tumor cells

 

Most cancers continues to be a significant public-health drawback that threatens human life worldwide and additional research must be carried out within the primary and preclinical areas. Though high-throughput sequencing expertise and individualized exact remedy have made breakthroughs over time, the excessive failure fee of medical translational analysis has restricted the innovation of antitumor medication and triggered the pressing want for optimum cancer-research fashions.

The event of cancerous cell traces, patient-derived xenograft (PDX) fashions, and organoid has strongly promoted the event of tumor-biology analysis, however the prediction values are restricted.

Conditional reprogramming (CR) is a novel cell-culture technique for most cancers analysis combining feeder cells with a Rho-associated coiled-coil kinase (ROCK) inhibitor, which permits the fast and steady proliferation of major epithelial cells.

On this evaluation, we summarize the methodology to ascertain CR mannequin and overview latest features and functions of CR cell-culture fashions in most cancers analysis with regard to the research of cancer-biology characterization, the exploration of therapeutic targets, individualized drug screening, the illumination of mechanisms about response to antitumor medication, and the development of patient-derived animal fashions, and at last focus on intimately the most important limitations of this cell-culture system.

 

 

Immortalized Human Oral Keratinocytes

T0630 1x106 cells / 1.0 ml
EUR 3950

Primary Mouse Neonatal Epidermal Keratinocytes (NEK)

CSI075Mu01 5X10^5
EUR 940

Primary Rabbit Neonatal Epidermal Keratinocytes (NEK)

CSI075Rb01 5X10^5
EUR 1360

Immortalized Human Oral Keratinocytes-hTERT

CSC-I9230L One Frozen vial Ask for price

Immortalized Mouse Oral Keratinocytes (IMOK)

T0855 1x10^6 cells / 1.0 ml
EUR 3950

Immortalized Normal Human Oral Mucosal Keratinocytes

T0768 5x10^5 cells / 1.0 ml
EUR 3950

Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM)

T0717 1x10^6 cells / 1.0 ml
EUR 3950

Human Skin PrimaCell1: Normal Epidermal Keratinocytes

2-96101 1 Kit Ask for price

Human Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes

2-96052 1 Kit Ask for price

Human Keratinocytes

ALHE41 1ml frozen Vial
EUR 620

Human Skin PrimaCell1: Normal Epidermal Keratinocytes Growth Medium

9-46101 5 x 100 ml Ask for price

Human New Borns PrimaCell: Normal New Born Epidermal Keratinocytes

2-96093 1 Kit Ask for price

Human Keratinocytes, Adult

ABC-TC3030 1 vial Ask for price
Description: Gentaur epidermal keratinocytes are available from adult and neonatal donor tissue and are provided at the earliest passage without the use of a murine feeder layer. Keratinocytes are available from donors of varying ages, genders, and diabetes status. Donor matched epidermal keratinocytes, dermal fibroblasts, melanocytes and subcutaneous preadipocytes are available.

Human keratinocytes, Dermis

ABC-TC3659 1 vial Ask for price
Description: The HKER-D cultures are produced on the base of human tissues. The HKER-D are isolated according to known procedures. Then primary cultures are produced.

Human Dermal Keratinocytes

cAP-0212 1Frozen Vial
EUR 475.2

Rat Skin PrimaCell1: Normal Epidermal Keratinocytes

2-82582 1 Kit Ask for price

Human Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes Growth Medium

9-46052 5 x 100 ml Ask for price

Human keratinocytes, Foreskin

ABC-TC3660 1 vial Ask for price
Description: The HKER-F cultures are produced on the base of human tissues. The HKER-F are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HKER-F are frozen in a computer controlled freezing unit.

Human Gingival Keratinocytes

cAP-0216 1Frozen Vial
EUR 643.5

Mouse Skin PrimaCell1: Normal Epidermal Keratinocytes

2-82085 1 Kit Ask for price

Human Caucasian dysplastic oral keratinocyte

P0020024 One Frozen vial
EUR 995

Human New Borns PrimaCell: Normal New Born Epidermal Keratinocytes Growth Medium

9-46093 5 x 100 ml Ask for price

Human Corneal Keratinocytes (HCK)

P0029012 One Frozen vial
EUR 650

Rat Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes

2-82533 1 Kit Ask for price

Mouse Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes

2-82032 1 Kit Ask for price

Human iPSC-derived Keratinocytes

CSC-00840L One Frozen vial Ask for price

Human Epidermal Keratinocytes, Adult

ABC-TC3604 1 vial Ask for price
Description: The epithelial layer of the skin provides an essential function as a protective barrier against insult from the outside environment. The major cell type in this layer is keratinocytes which consists around 85% of living epidermal cells. Keratinocytes are the cells of stratified squamous epithelia. It is so named because its most abundant protein is keratin. Keratinocyte divide and are thus generated in the basal layer of the epidermis; this division is followed by a programmed death as they produce keratin and move up to the surface of the epidermis. Keratinocyte proliferation, differentiation, and apoptosis are complex and carefully choreographed processes. Besides the protective function, keratinocytes also play a central role in tissue homeostasis, wound healing, cancers, and skin-based gene-therapy. Human keratinocytes express adhesion molecules and cytokines indicating their participation in skin innate immunity and homeostasis in vivo. HEK-a from Gentaur Research Laboratories are isolated from adult human epidermal tissue. HEK-a are cryopreserved on primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEK-a are characterized by immunofluorescent method with antibodies to cytokeratine-18 and -19. HEK-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEK-a are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Epidermal Keratinocytes, Fetal

ABC-TC3605 1 vial Ask for price
Description: The epithelial layer of the skin provides an essential function as a protective barrier against insult from the outside environment. The major cell type in this layer is keratinocytes which consists around 85% of living epidermal cells. Keratinocytes are the cells of stratified squamous epithelia. It is so named because its most abundant protein is keratin. Keratinocyte divide and are thus generated in the basal layer of the epidermis; this division is followed by a programmed death as they produce keratin and move up to the surface of the epidermis. Keratinocyte proliferation, differentiation, and apoptosis are complex and carefully choreographed processes. Besides the protective function, keratinocytes also play a central role in tissue homeostasis, wound healing, cancers, and skin-based gene-therapy. Human keratinocytes express adhesion molecules and cytokines indicating their participation in skin innate immunity and homeostasis in vivo. HEK-f from Gentaur Research Laboratories are isolated from fetal human epidermal tissue. HEK-f are cryopreserved on primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEK-f are characterized by immunofluorescent method with antibodies to cytokeratine-18 and -19. HEK-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEK-f are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Hair Follicular Keratinocytes

ABC-TC3637 1 vial Ask for price
Description: Hair follicles are self-renewing structures that cycle and reconstitute themselves throughout life because they contain keratinocyte stem cells. The interplay between keratinocytes and underlying mesenchyme are critical for hair follicle morphogenesis and cycling. Several key developmental pathways are involved in determining follicular keratinocyte fate as well as the differentiation into several distinct cellular layers of the follicle during the growth phase of hair. Studies indicate that follicular keratinocytes are distinct from epidermal keratinocytes in proliferative capacity and CD34 expression.HHFK from Gentaur Research Laboratories are isolated from human hair follicles. HHFK are cryopreserved on primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HHFK are characterized by immunofluorescent method with antibodies to cytokeratines. HHFK are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHFK are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Keratinocytes, Pooled Neonatal

ABC-TC3662 1 vial Ask for price
Description: Gentaur epidermal keratinocytes are available from adult and neonatal donor tissue and are provided at the earliest passage without the use of a murine feeder layer. Keratinocytes are available from donors of varying ages, genders, and diabetes status. Donor matched epidermal keratinocytes, dermal fibroblasts, melanocytes and subcutaneous preadipocytes are available.

Human Hair Follicular Keratinocytes 

CSC-7776W One Frozen vial Ask for price

Human Epidermal Keratinocytes, Foetal

ABC-TC3603 1 vial Ask for price
Description: Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.

Normal Human Epidermal Keratinocytes 

CSC-7640W One Frozen vial Ask for price

Human Dermal Epidermal Keratinocytes

CSC-C9225J One Frozen vial Ask for price

Rat New Borns PrimaCell: Normal New Born Epidermal Keratinocytes

2-82575 1 Kit Ask for price

Rat Skin PrimaCell1: Normal Epidermal Keratinocytes Growth Medium

9-25082 5 x 100 ml Ask for price

Human Epidermal Keratinocytes, Neonatal

ABC-TC3606 1 vial Ask for price
Description: The epithelial layer of the skin provides an essential function as a protective barrier against insult from the outside environment. The major cell type in this layer is keratinocytes which consists around 85% of living epidermal cells. Keratinocytes are the cells of stratified squamous epithelia. It is so named because its most abundant protein is keratin. Keratinocyte divide and are thus generated in the basal layer of the epidermis; this division is followed by a programmed death as they produce keratin and move up to the surface of the epidermis. Keratinocyte proliferation, differentiation, and apoptosis are complex and carefully choreographed processes. Besides the protective function, keratinocytes also play a central role in tissue homeostasis, wound healing, cancers, and skin-based gene-therapy. Human keratinocytes express adhesion molecules and cytokines indicating their participation in skin innate immunity and homeostasis in vivo. HEK-n from Gentaur Research Laboratories are isolated from neonate human epidermal tissue. HEK-n are cryopreserved on primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEK-n are characterized by immunofluorescent method with antibodies to cytokeratine-18 and -19. HEK-n are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEK-n are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Neonatal Epidermal Keratinocytes

ABC-TC3985 1 vial Ask for price
Description: Neonatal Human Epidermal Keratinocytes (NHEK) are cultured by first, isolating the keratinocytes from the epidermis of the neonatal skin and then, establishing a primary keratinocyte culture. Upon completion of the primary culture, the cells are harvested, aliquoted into individual vials, and placed in liquid nitrogen storage until shipment. This product has been thoroughly tested and found to be free of the HIV Type I, Viral Hepatitis Type B and C, mycoplasma, bacteria, yeast, and fungi.

Immortalized Human Dermal Keratinocytes

CSC-I2035Z One Frozen vial Ask for price

Mouse New Borns PrimaCell: Normal New Born Epidermal Keratinocytes

2-82076 1 Kit Ask for price

Mouse Skin PrimaCell1: Normal Epidermal Keratinocytes Growth Medium

9-32085 5 x 100 ml Ask for price

Immortalized Human Epidermal Keratinocytes

T0345 1x106 cells / 1.0 ml
EUR 3950

Human Keratinocytes, Adult, Diabetic Donor

ABC-TC3031 1 vial Ask for price
Description: Gentaur epidermal keratinocytes are available from adult and neonatal donor tissue and are provided at the earliest passage without the use of a murine feeder layer. Keratinocytes are available from donors of varying ages, genders, and diabetes status. Donor matched epidermal keratinocytes, dermal fibroblasts, melanocytes and subcutaneous preadipocytes are available.

Human Keratinocytes, Neonatal, Single Donor

ABC-TC3661 1 vial Ask for price
Description: Gentaur epidermal keratinocytes are available from adult and neonatal donor tissue and are provided at the earliest passage without the use of a murine feeder layer. Keratinocytes are available from donors of varying ages, genders, and diabetes status. Donor matched epidermal keratinocytes, dermal fibroblasts, melanocytes and subcutaneous preadipocytes are available.

Human Epidermal Keratinocytes, Adult, Pooled

ABC-TC3976 1 vial Ask for price
Description: Epidermal keratinocytes represent the major cell type of the epidermis, making up about 90% of the cells. They originate in the stratum basale and undergo gradual differentiation including profound morphological changes during their shift to the stratum corneum. In the stratum corneum, the final barrierlayer of the skin, keratinocytes are found as nucleus-free, flat, and highly keratinized squamous cells. Gentaur offers a range of Normal Human Epidermal Keratinocytes (NHEK) from single donors or pooled donors produced at Promo- Cell’s cell culture facility. The cells are isolated from juvenile foreskin or from adult normal human tissue from different locations, e.g. face, breast, abdomen, and thighs. Shortly after isolation, all Gentaur Normal Human Epidermal Keratinocytes are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Skin PrimaCell1: Normal Epidermal Keratinocytes Growth Supplements with Serum (for 500 ml medium)

9-47101 1 Set Ask for price

Rat Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes Growth Medium

9-25033 5 x 100 ml Ask for price

Human Epidermal Keratinocytes, Neonatal, Pooled

ABC-TC3961 1 vial Ask for price
Description: Normal Human Epidermal Keratinocytes (NHEK) have longer guaranteed life spans than KGM or KGM-2 cells with ≥18 total population doublings for Adult cells and ≥20 population doublings for neonatal cells.

Immortalized Human Corneal Keratinocytes (IHCK)

T0579 1x106 cells / 1.0 ml
EUR 3950

Immortalized Human Epidermal Keratinocytes (SIK)

T0770 1x106 cells / 1.0 ml
EUR 3950

Mouse Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes Growth Medium

9-32032 5 x 100 ml Ask for price

Human Epidermal Keratinocytes, 10-Donor Pool

ABC-TC3980 1 vial Ask for price
Description: Normal Human Epidermal Keratinocytes (HEK), when grown in LIDer K Medium, provide an ideal serum-free culture model, for the study of wound healing, toxicology or epithelial biology. When in need of multiple keratinocyte cell lots, our HEK, 10-donor pool offers a wide range of donor demographics for research at an economical price. All HEK are quality tested in LIDer K Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Our HEK need not be exposed to antimicrobials or phenol red when cultured in LIDer medium; an advantage since these supplements can cause cell stress and "masking effects" that may negatively impact experimental result.Cell Features:HEKn are cryopreserved as primary cells. Cells are isolated from neonatal human foreskin and expanded once in culture vessels before cryopreservation.HEKa are cryopreserved as primary cells. Cells are isolated from adult human skin and expanded once in culture vessels before cryopreservation.HEK can be grown without serum, phenol red or antimicrobials when cultured in DermaLife medium.HEK are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Rat New Borns PrimaCell: Normal New Born Epidermal Keratinocytes Growth Medium

9-25075 5 x 100 ml Ask for price

SV40-Human Dermal Keratinocytes

cAP-0212-SV40 1Frozen Vial
EUR 5049

hTert-Human Dermal Keratinocytes

cAP-0212-hTert 1Frozen Vial
EUR 5049

Immortalized Human Epidermal Keratinocytes-SV40

CSC-I9096L One Frozen vial Ask for price

Immortalized Human Gingival Keratinocytes-hTERT

CSC-I9236L One Frozen vial Ask for price

Mouse New Borns PrimaCell: Normal New Born Epidermal Keratinocytes Growth Medium

9-32076 5 x 100 ml Ask for price

SV40-Human Gingival Keratinocytes

cAP-0216-SV40 1Frozen Vial
EUR 5049

Human Embryo PrimaCell3: Normal Fetal Epidermal Keratinocytes Growth Supplements with Serum (for 500 ml medium)

9-47052 1 Set Ask for price

Immortalized Human Epidermal Keratinocytes-hTERT

CSC-I2104Z One Frozen vial Ask for price

Human Epidermal Keratinocytes, Adult, Single Donor

ABC-TC3977 1 vial Ask for price
Description: Epidermal keratinocytes represent the major cell type of the epidermis, making up about 90% of the cells. They originate in the stratum basale and undergo gradual differentiation including profound morphological changes during their shift to the stratum corneum. In the stratum corneum, the final barrierlayer of the skin, keratinocytes are found as nucleus-free, flat, and highly keratinized squamous cells. Gentaur offers a range of Normal Human Epidermal Keratinocytes (NHEK) from single donors or pooled donors produced at Promo- Cell’s cell culture facility. The cells are isolated from juvenile foreskin or from adult normal human tissue from different locations, e.g. face, breast, abdomen, and thighs. Shortly after isolation, all Gentaur Normal Human Epidermal Keratinocytes are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

hTert-Human Gingival Keratinocytes

cAP-0216-hTert 1Frozen Vial
EUR 5049

Rat Epidermal Keratinocytes

ABC-TC4130 1 vial Ask for price
Description: Rat Epidermal Keratinocytes (REK) are derived from the skin of normal Sprague Dawley rats. They are cryopreserved at the end of primary culture. These epithelial cells proliferate well when thawed and plated from cryopreservation.

Human Adult Epi. Keratinocytes-Diabetic Type II

ABC-TC3481 1 vial Ask for price
Description: Diseased Human Epidermal Keratinocytes (D-HEK) are isolated from human donors that have been diagnosed with diabetes type II disease. Diseased cells are grown in the same optimized media system as Normal Human Keratinocytes.

Human New Borns PrimaCell: Normal New Born Epidermal Keratinocytes Growth Supplements with Serum (for 500 ml medium)

9-47093 1 Set Ask for price

Human HACAT Keratinocyte Cells

CR1017-500 1 vial
EUR 1000
Description: Cryopreserved vial of human keratinocyte cells containing ≥ 500,000 cells

Human Epidermal Keratinocytes, Juvenile Foreskin, Pooled

ABC-TC3978 1 vial Ask for price
Description: Epidermal keratinocytes represent the major cell type of the epidermis, making up about 90% of the cells. They originate in the stratum basale and undergo gradual differentiation including profound morphological changes during their shift to the stratum corneum. In the stratum corneum, the final barrierlayer of the skin, keratinocytes are found as nucleus-free, flat, and highly keratinized squamous cells. Gentaur offers a range of Normal Human Epidermal Keratinocytes (NHEK) from single donors or pooled donors produced at Promo- Cell’s cell culture facility. The cells are isolated from juvenile foreskin or from adult normal human tissue from different locations, e.g. face, breast, abdomen, and thighs. Shortly after isolation, all Gentaur Normal Human Epidermal Keratinocytes are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Immortalized Human Foreskin Keratinocytes (HFK-16E6E7)

T0723 1x10^6 cells / 1.0 ml
EUR 3950

Immortalized Human Gingival Keratinocytes (Gie-No3B11)

T0049 1x106 cells / 1.0 ml Ask for price

hTert-CDK4-Human Dermal Keratinocytes

cAP-0212-hTert-CDK4 1Frozen Vial
EUR 6435

NHEK-Neo, Human Epidermal Keratinocytes, Neonatal, Pooled

LO192906 each
EUR 480.01