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Device for Ammonia Detection in Cell Culture Media

Device for Ammonia Detection in Cell Culture Media

Excessive-Facet-Ratio SU-8-Based mostly Optofluidic System for Ammonia Detection in Cell Tradition Media

 

Miniaturization of sensing expertise has led to the event of multifunctional micro whole evaluation techniques (µTAS) that profit from microfluidics expertise. Optical sensing is without doubt one of the mostly used sensing approaches built-in into µTAS gadgets and options excessive sensitivity and low detection limits.

    • Totally different supplies have been used for the fabrication of µTAS gadgets every having their benefit and downsides. Herein, a high-aspect-ratio optofluidic waveguide fabricated from SU-Eight is offered for the primary time.

 

    • The appropriate optical properties and chemical inertness of SU-Eight present a sturdy gadget made by a versatile and cost-efficient fabrication course of.

 

    • The optofluidic gadget was used for colorimetric ammonia (NH3) sensing with a dynamic vary of 3-70 µM, a detection restrict of two.5 µM, a response time of Eight min, and near 10 instances higher analytical efficiency in comparison with utilizing a normal microplate reader.

 

    • The µTAS gadget was able to monitoring NH3 accumulating within the cell tradition media of prostatic epithelial cell (BPH-1) tradition.
Lentivectors
Lentivectors

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47009 1 Set Ask for price

Rat Bladder Tissue Preparation Buffer 3: Normal Smooth Muscle Cells

9-80215 1 x 100 ml Ask for price

Human Bladder Smooth Muscle Cells

ABC-TC3514 1 vial Ask for price
Description: Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all the same types of filaments, but, depending on the tissue of origin, differ significantly in mechanical and physiological properties. Therefore, Gentaur offers a range of Human Smooth Muscle Cells produced at Gentaur's cell culture facility from normal human tissue of different origins. The cells are isolated from the aorta, the coronary artery, the pulmonary artery, the umbilical artery,the trachea, the bronchi, the bladder,the prostate, and the uterus. Shortly after isolation, all Gentaur Human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Primary Bladder Smooth Muscle Cells

T4091 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Bladder Smooth Muscle Cells

P0002010 One Frozen vial
EUR 600

Rat Bladder Smooth Muscle Cells

ABC-TC4082 1 vial Ask for price
Description: Rat bladder smooth muscle cells, 3-week Wistar rat

Rat Bladder Smooth Muscle Cells

CP-R059 5×105 cells/vial
EUR 580
Description: Rat

Mouse Bladder Smooth Muscle Cells

CP-M059 5×105 cells/vial
EUR 580
Description: Mouse

Rabbit Bladder Smooth Muscle Cells

CP-Rb020 5×105 cells/vial
EUR 640
Description: Rabbit

Immortalized Human Bladder Smooth Muscle Cells

CSC-I9126L One Frozen vial Ask for price

Immortalized Human Urinary Bladder Smooth Muscle Cells

T0617 1x106 cells / 1.0 ml
EUR 2750

Rat Bladder PrimaCell3: Normal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-26008 1 Set Ask for price

Primary Rabbit Bladder Smooth Muscle Cells (BSMC)

CSI065Rb01 5X10^5
EUR 920

Rat Bladder Smooth Muscle Cells Complete Medium

CM-R059-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rat Bladder Smooth Muscle Cells Complete Medium

CM-R059-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Rat Primary Urinary Bladder Smooth Muscle Cells

T4937 5x10^5 cells / 1.0 ml Ask for price

Rat Bladder Smooth Muscle Cells Complete Medium

CM-R059 100mL
EUR 98
Description: Primary cells complete growth medium

Mouse Bladder Smooth Muscle Cells Complete Medium

CM-M059-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Mouse Bladder Smooth Muscle Cells Complete Medium

CM-M059-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Mouse Bladder Smooth Muscle Cells Complete Medium

CM-M059 100mL
EUR 98
Description: Primary cells complete growth medium

Rabbit Bladder Smooth Muscle Cells Complete Medium

CM-Rb020-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rabbit Bladder Smooth Muscle Cells Complete Medium

CM-Rb020-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Rabbit Bladder Smooth Muscle Cells Complete Medium

CM-Rb020 100mL
EUR 98
Description: Primary cells complete growth medium

Immortalized Mouse Bladder Smooth Muscle Cells-SV40

CSC-I2171Z One Frozen vial Ask for price

Immortalized Canine Bladder Smooth Muscle Cells-SV40

CSC-I2131Z One Frozen vial Ask for price

Human Muscle PrimaCell2: Normal Smooth Muscle Cells

2-96092 1 Kit Ask for price

Rat Muscle PrimaCell2: Normal Smooth Muscle Cells

2-82574 1 Kit Ask for price

Mouse Muscle PrimaCell2: Normal Smooth Muscle Cells

2-82075 1 Kit Ask for price

Human Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium

9-46092 5 x 100 ml Ask for price

Human Heart PrimaCell1: Normal Aortic Smooth Muscle Cells

2-96066 1 Kit Ask for price

Human Rectal PrimaCell: Normal Rectal Smooth Muscle Cells

2-96100 1 Kit Ask for price

Human Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells

9-80092 1 x 100 ml Ask for price

Human Cervix PrimaCell3: Normal Cervical Smooth Muscle Cells

2-96046 1 Kit Ask for price

Human Esophagus PrimaCell2: Normal Esophageal Smooth Muscle Cells

2-96056 1 Kit Ask for price

Human Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells

2-96072 1 Kit Ask for price

Rat Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium

9-25074 5 x 100 ml Ask for price

Human Lung PrimaCell6: Normal Pulmonary Vein Smooth Muscle Cells

2-96086 1 Kit Ask for price

Mouse Muscle PrimaCell2: Normal Smooth Muscle Cells Growth Medium

9-32075 5 x 100 ml Ask for price

Human Lung PrimaCell4: Normal Pulmonary Artery Smooth Muscle Cells

2-96084 1 Kit Ask for price

Rat Heart PrimaCell1: Normal Aortic Smooth Muscle Cells

2-82546 1 Kit Ask for price

Rat Rectal PrimaCell: Normal Rectal Smooth Muscle Cells

2-82581 1 Kit Ask for price

Rat Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells

9-80281 1 x 100 ml Ask for price

Mouse Heart PrimaCell1: Normal Aortic Smooth Muscle Cells

2-82045 1 Kit Ask for price

Mouse Rectal PrimaCell: Normal Rectal Smooth Muscle Cells

2-82084 1 Kit Ask for price

Mouse Muscle Tissue Preparation Buffer 2: Normal Smooth Muscle Cells

9-80189 1 x 100 ml Ask for price

Rat Cervix PrimaCell3: Normal Cervical Smooth Muscle Cells

2-82526 1 Kit Ask for price

Rat Colon PrimaCell2: Normal Colorectal Smooth Muscle Cells

2-82530 1 Kit Ask for price

Human Heart PrimaCell1: Normal Aortic Smooth Muscle Cells Growth Medium

9-46066 5 x 100 ml Ask for price

Human Rectal PrimaCell: Normal Rectal Smooth Muscle Cells Growth Medium

9-46100 5 x 100 ml Ask for price

Mouse Cervix PrimaCell3: Normal Cervical Smooth Muscle Cells

2-82025 1 Kit Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-96007 1 Kit Ask for price

Human Rectal Tissue Preparation Buffer: Normal Rectal Smooth Muscle Cells

9-80100 1 x 100 ml Ask for price

Rat Esophagus PrimaCell2: Normal Esophageal Smooth Muscle Cells

2-82537 1 Kit Ask for price

Rat Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells

2-82553 1 Kit Ask for price

Human Airway PrimaCell3: Normal Bronchial and Tracheal Smooth Muscle Cells

2-96003 1 Kit Ask for price

Human Brain PrimaCell3: Normal Cerebral Artery Vascular Smooth Muscle Cells

2-96015 1 Kit Ask for price

Human Brain PrimaCell6: Normal Cerebral Venous Vascular Smooth Muscle Cells

2-96018 1 Kit Ask for price

Human Umbilical Cord PrimaCell5: Normal Umbilical Vein Smooth Muscle Cells

2-96110 1 Kit Ask for price

Human Cervix PrimaCell3: Normal Cervical Smooth Muscle Cells Growth Medium

9-46046 5 x 100 ml Ask for price

Mouse Esophagus PrimaCell2: Normal Esophageal Smooth Muscle Cells

2-82036 1 Kit Ask for price

Mouse Intestine PrimaCell4: Normal Intestinal Smooth Muscle Cells

2-82054 1 Kit Ask for price

Human Umbilical Cord PrimaCell2: Normal Umbilical Artery Smooth Muscle Cells

2-96107 1 Kit Ask for price

Rat Lung PrimaCell6: Normal Pulmonary Vein Smooth Muscle Cells

2-82569 1 Kit Ask for price

Human Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells

9-80066 1 x 100 ml Ask for price

Human Esophagus PrimaCell2: Normal Esophageal Smooth Muscle Cells Growth Medium

9-46056 5 x 100 ml Ask for price

Human Intestine PrimaCell2: Normal Intestinal Smooth Muscle Cells Growth Medium

9-46072 5 x 100 ml Ask for price

 

Vast floor pore microfiltration membrane drastically improves sieving decay in TFF-based perfusion cell tradition and streamline chromatography integration for steady bioprocessing

Though a number of compelling advantages for bioprocess intensification have been reported, the necessity for a streamlined integration of perfusion cultures with seize chromatography nonetheless stays unmet. Right here, a sturdy resolution is established by conducting TFF-based perfusion with a large floor pore microfiltration membrane.

The ensuing built-in steady bioprocess demonstrated negligible retention of antibody, DNA and host cell proteins within the bioreactor with common sieving coefficients of 98±1%, 124±28% and 109±27%, respectively. Additional dialogue relating to the potential membrane fouling mechanisms can be supplied by evaluating two membranes with totally different floor pore buildings and the identical hole fiber size, whole membrane space, and chemistry.

A cake-growth profile is reported for the narrower floor pore, 0.65-µm nominal retention perfusion membrane with closing antibody sieving coefficients ≤70%. Whereas the sieving coefficient remained ≥85% throughout 40 tradition days for the huge floor pore, 0.2-µm nominal retention score membrane.

The huge floor pore construction, confirmed by SEM imaging, minimizes the formation of biomass deposits on the membrane floor and drastically improves product sieving. This work not solely affords a sturdy different for built-in steady bioprocess by eliminating further filtration steps whereas overcoming sieving decay, however it additionally gives perception into the membranes’ fouling mechanism. This text is protected by copyright. All rights reserved.

 

Anti-Inflammatory and Barrier-Stabilising Results of Myrrh, Espresso Charcoal and Chamomile Flower Extract in a Co-Tradition Cell Mannequin of the Intestinal Mucosa

 

Latest scientific proof suggests the efficacy of a standard natural medicinal product containing myrrh (Commiphora molmol Engl.), espresso charcoal (Coffea arabica L.) and chamomile flower dry extract (Matricaria chamomilla L.) within the remedy of inflammatory bowel ailments (IBD).

 

    • Nonetheless, the mechanisms of motion on this context haven’t been solely elucidated. The current research aimed to guage the results of myrrh, espresso charcoal and chamomile flower extract on the inflammatory cross speak between immune and intestinal epithelial cells along with the ensuing intestinal barrier problems.

 

    • A fancy co-culture cell mannequin consisting of intestinal epithelial cell (IEC) monolayers (Caco-2, HT29-MTX-E12) and macrophages (THP-1) was established for the simultaneous investigation of those two IBD traits.

 

    • The lipopolysaccharide (LPS) activation of the macrophages led to a pro-inflammatory mediator launch and thereby an inflammatory stimulation of IECs with chemokine launch and decreased barrier operate.

 

    • The results of the person plant extracts and a ternary mixture on inflammatory mediator launch (IL-6, TNF, IL-8, MCP-1, PGE2) was quantified by ELISA.

 

    • The transepithelial electrical resistance (TEER) of IEC monolayers was measured to guage the results on the barrier operate. Budesonide served as a constructive management.

 

    • All three plant extracts exhibited anti-inflammatory properties through the inhibition of the inflammatory mediator launch to a various extent.

 

    • An intestinal barrier stabilising impact was noticed for myrrh and occasional charcoal. Myrrh exerted probably the most distinct pharmacological exercise.

 

    • Dose decreasing and synergistic interactions emerged throughout the threefold mixture. Thus, our outcomes present a mechanistic foundation for using the natural mixture of myrrh, espresso charcoal and chamomile flower extract in IBD therapy and underline the potential advantages of the phytotherapeutic multi-component/multi-target method on this complicated pathogenesis.

 

Key phrases: IBD; chamomile flower; co-culture cell mannequin; espresso charcoal; irritation; intestinal barrier; mucosa; myrrh.

 

Bovine Lingual antimicrobial peptide (LAP3)

1-CSB-EP632987BO
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bovine Lingual antimicrobial peptide(LAP3),partial expressed in E.coli

Human Cryopreserved Bone Cells, Normal

CDD-H-2810-N 1 vial
EUR 600

Recombinant Bovine Lingual antimicrobial peptide(LAP)

AP71306 1mg
EUR 2826

Bovine Lingual antimicrobial peptide, LAP ELISA KIT

ELI-36982b 96 Tests
EUR 1113.6

Human Fat PrimaCell1: Normal Adipose Cells

2-96062 1 Kit Ask for price

Recombinant Bubalus bubalis Lingual antimicrobial peptide

CSB-EP389004BWX 3507 mg Ask for price

Recombinant Bubalus bubalis Lingual antimicrobial peptide

RPC27691-100ug 100ug
EUR 801.9

Recombinant Bubalus bubalis Lingual antimicrobial peptide

RPC27691-1mg 1mg
EUR 2885.2

Recombinant Bubalus bubalis Lingual antimicrobial peptide

RPC27691-20ug 20ug
EUR 448.1

Normal Human Small Airway Epithelial Cells

T5514 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Kidney Cortical Cells

T5517 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Hepatic Kupffer Cells

T5549 1x10^6 cells / 1.0 ml
EUR 1115

Human Testis PrimaCell: Normal Sertoli Cells

2-96105 1 Kit Ask for price

Normal Human Primary Kidney Mesangial Cells

T5521 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Hepatic Stellate Cells

T5546 1x10^6 cells / 1.0 ml
EUR 1175

Human Brain PrimaCell7: Normal Meningeal Cells

2-96019 1 Kit Ask for price

Human Normal Peripheral Blood CD56+ NK Cells

PBCD56P-C5M 5 million
EUR 825.6

Human Normal Peripheral Blood CD56+ NK Cells

PBCD56U-C5M 5 million
EUR 1014

Human Normal Peripheral Blood CD56+ NK Cells

PBCD56U-F5M 5 million
EUR 1198.8

Normal Human Primary Broncial Epithelial Cells

T5511 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Tracheal Epithelial Cells

T5512 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Tracheal Epithelial Cells

T5513 5x10^5 cells / 1.0 ml
EUR 875

Human Lymph PrimaCell1: Normal Endothelial Cells

2-96087 1 Kit Ask for price

Normal Human Primary Bronchial Epithelial Cells

T5510 5x10^5 cells / 1.0 ml
EUR 875

Human Embryo PrimaCell4: Normal Trophoblast Cells

2-96053 1 Kit Ask for price

HighQC™ Normal Human Pre-Adipocyte Cells

ABC-SC0100T 1 vial Ask for price
Description: Human Pre-Adipocyte Cells provide an ideal culture model for the study of diabetes, obesity, metabolism, RI sensitivity, and adipose biology.Pre-Adipocytes are derived from mature adipocytes that have been dedifferentiated, and are cryopreserved as secondary cells to ensure optimal phenotype and the highest viability and plating efficiency. Human Pre-Adipocytes can be expanded in an undifferentiated state for future differentiation to mature Adipocytes. Human Pre-Adipocytes may also be differentiated down chondrogenic, and osteogenic lineages. Human Pre-Adipocytes are characterized by flow cytometry to ensure the proper expression of multiple markers of mesenchymal stem cells. They are uniformly positive for CD29, CD44, CD73, CD90, CD105, and CD166. They are uniformly negative for CD14, CD31, CD34, and CD45. has developed optimized media to expand the Human Pre-Adipocyte Stem Cell products in the undifferentiated state, as well as optimized media kits for inducing Adipogenesis, Chondrogenesis, and Osteogenesis. Additionally, provides convenient staining kits for staining the fully differentiated cells.

Normal Human Primary Sinusoidal Endothelial Cells

T5558 1x10^6 cells / 1.0 ml
EUR 875

Human Fat PrimaCell4: Normal Visceral Fat Cells

2-96065 1 Kit Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells

PBCD3-C10M 10 million
EUR 562.8

Human Normal Peripheral Blood CD3+ Pan T Cells

PBCD3-C20M 20 million
EUR 853.2

Human Normal Peripheral Blood CD3+ Pan T Cells

PBCD3-F100M 100 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells

PBCD3-F20M 20 million
EUR 1002

Human Normal Peripheral Blood CD3+ Pan T Cells

PBCD3-F500M 500 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells

PBCD3-F50M 50 million
EUR 1798.8

Human Eye PrimaCell4: Normal Lens Epithelial Cells

2-96060 1 Kit Ask for price

Normal Human Primary Kidney Proximal Tubule Cells

T5520 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Peripheral Blood Mononuclear Cells, Frozen -100 million cells

79059-2 100 million cells
EUR 580
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-C100M 100 million
EUR 478.8

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-C10M 10 million
EUR 118.8

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-C20M 20 million
EUR 202.8

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-C50M 50 million
EUR 319.2

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-F100M 100 million
EUR 510

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-F1B 1 billion
EUR 2215.2

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-F2B 2 billion
EUR 4268.4

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-F300M 300 million
EUR 912

Human Normal Peripheral Blood Mononuclear Cells (PBMC)

PBMC-F500M 500 million
EUR 1366.8

Human Fat PrimaCell3: Normal Subcutaneous Fat Cells

2-96064 1 Kit Ask for price

Human Muscle PrimaCell2: Normal Smooth Muscle Cells

2-96092 1 Kit Ask for price

Normal Human Primary Small Airway Epithelial Cells

T5515 5x10^5 cells / 1.0 ml
EUR 875

Human Normal Peripheral Blood CD4+ T Helper Cells

PBCD4-C10M 10 million
EUR 718.8

Human Normal Peripheral Blood CD4+ T Helper Cells

PBCD4-F10M 10 million
EUR 790.8